Hormonal Regulation of Rat Granulosa Cell Deoxyribonucleic Acid Synthesis: Effects of Estrogen1
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چکیده
منابع مشابه
Inhibitory effect of gonadotrophin-releasing hormone (GnRH) on rat granulosa cell deoxyribonucleic acid synthesis.
Gonadotropin-releasing hormone (GnRH) has been found to be expressed within the ovary and to modulate cell differentiation in ovarian cells. In the present study we have analyzed the influence of GnRH on DNA synthesis in rat granulosa cells. Cells were obtained from immature DES-treated rats and cultured in defined medium (DMEM:F12) containing combinations of FSH, estradiol, and transforming gr...
متن کاملRegulation of Deoxyribonucleic Acid Synthesis
These enzymes (Grav, 1967) are responsible for the synthesis of the monomeric precursors of DNA, namely dATP, dCTP, dGTP and dTTP. Fluctuations in the activities of some of the enzymes and in the intracellular deoxyribonucleotide pools are known to occur throughout the cell cycle (Grav, 1967; Nordenskjold et al., 1970; Adams et al., 1971 ; Skoog & Nordenskjold, 1971). Moreover, there is inducti...
متن کاملTransient Phosphorylation of Deoxyribosides and Regulation of Deoxyribonucleic Acid Synthesis
Microspores isolated from Lilium longiflorum and Trillium erectum were studied with respect to their capacities for phosphorylating deoxyribosides in vitro. It was found that such capacities are manifest only during brief intervals of time adjacent to periods of DNA synthesis, and that none of the neighboring cells in the anther acquire them. The observed patterns of behavior are interpreted in...
متن کاملEnzymatic Synthesis of Deoxyribonucleic Acid. Deoxyribonucleic Acid By
MISSOURI The enzymatic synthesis of DNA1 in sufficient excess over the DNA used to prime the reaction21 a has enabled us to examine the chemical composition of the synthetic product. As has already been demonstrated, this DNA consists of deoxy-nucleotides Linked by typical 3'-5' phosphodiester bonds, and all four of the deoxy
متن کاملEnzymatic synthesis of deoxyribonucleic acid.
Determination of deoxyribonucleotide sequence in a deoxyribonucleic acid molecule is important from both the chemical and genetic points of view. It is also essential for answering the question of whether DNA synthesized in vitro by polymerase (1, 2) is a faithful copy of the nucleotidel sequence of the primer DNA. Although enzymatically synthesized DNA has the same over-all nucleotide composit...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1991
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod44.5.880